Poly(A) tail shortening by a mammalian poly(A)-specific 3'-exoribonuclease
- PMID: 9099687
- DOI: 10.1074/jbc.272.16.10448
Poly(A) tail shortening by a mammalian poly(A)-specific 3'-exoribonuclease
Abstract
3'-Exonucleolytic removal of the poly(A) tail is the first and often rate-limiting step in the decay of many eucaryotic mRNAs. In a cytoplasmic extract from HeLa cells, the poly(A) tail of mRNA was degraded from the 3'-end. In agreement with earlier in vivo observations, prominent decay intermediates differed in length by about 30 nucleotides. The Mg2+-dependent, poly(A)-specific 3'-exoribonuclease responsible for this poly(A) shortening activity was purified from calf thymus. A polypeptide of 74 kDa copurified with the activity. The deadenylating nuclease (DAN) required a free 3'-OH group, released solely 5'-AMP, degraded RNA in a distributive fashion, and preferred poly(A) as a substrate. At low salt concentration, the activity of purified DAN was strongly dependent on spermidine or other, yet unidentified factors. Under these reaction conditions, DAN was also stimulated by the cytoplasmic poly(A)-binding protein I (PAB I). At physiological salt concentration, the stimulatory effect of spermidine was weak and PAB I was inhibitory. At either salt concentration DAN and PAB I reconstituted poly(A) shortening with the same pattern of intermediates seen in cytoplasmic extract. The properties of DAN suggest that the enzyme might be involved in the deadenylation of mRNA in vivo.
Similar articles
-
3'-UTR-dependent deadenylation by the yeast poly(A) nuclease.Genes Dev. 1992 Nov;6(11):2088-99. doi: 10.1101/gad.6.11.2088. Genes Dev. 1992. PMID: 1358757
-
The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus oocytes.EMBO J. 1998 Sep 15;17(18):5427-37. doi: 10.1093/emboj/17.18.5427. EMBO J. 1998. PMID: 9736620 Free PMC article.
-
Cap-dependent deadenylation of mRNA.EMBO J. 2000 Mar 1;19(5):1079-86. doi: 10.1093/emboj/19.5.1079. EMBO J. 2000. PMID: 10698948 Free PMC article.
-
Modulation of poly(A)(+)mRNA-metabolizing and transporting systems under special consideration of microtubule protein and actin.Mol Cell Biochem. 1983;53-54(1-2):197-220. doi: 10.1007/BF00225254. Mol Cell Biochem. 1983. PMID: 6137761 Review.
-
The poly(A) tail of mRNAs: bodyguard in eukaryotes, scavenger in bacteria.Cell. 2002 Nov 27;111(5):611-3. doi: 10.1016/s0092-8674(02)01137-6. Cell. 2002. PMID: 12464173 Review.
Cited by
-
ProFAT: a web-based tool for the functional annotation of protein sequences.BMC Bioinformatics. 2006 Oct 23;7:466. doi: 10.1186/1471-2105-7-466. BMC Bioinformatics. 2006. PMID: 17059594 Free PMC article.
-
mRNA COVID-19 Vaccines-Facts and Hypotheses on Fragmentation and Encapsulation.Vaccines (Basel). 2022 Dec 24;11(1):40. doi: 10.3390/vaccines11010040. Vaccines (Basel). 2022. PMID: 36679885 Free PMC article.
-
Nerve growth factor controls GAP-43 mRNA stability via the phosphoprotein ARPP-19.Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12427-31. doi: 10.1073/pnas.152457399. Epub 2002 Sep 9. Proc Natl Acad Sci U S A. 2002. PMID: 12221279 Free PMC article.
-
Deciphering the cellular pathway for transport of poly(A)-binding protein II.RNA. 2000 Feb;6(2):245-56. doi: 10.1017/s1355838200991908. RNA. 2000. PMID: 10688363 Free PMC article.
-
UBP1, a novel hnRNP-like protein that functions at multiple steps of higher plant nuclear pre-mRNA maturation.EMBO J. 2000 Apr 3;19(7):1638-49. doi: 10.1093/emboj/19.7.1638. EMBO J. 2000. PMID: 10747031 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous
