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. 2013 Jan;5(1):308-314.
doi: 10.3892/etm.2012.777. Epub 2012 Oct 30.

Expression of intestinal transporter genes in beagle dogs

Soo-Min Cho  1 , Sung-Won Park, Na-Hyun Kim, Jin-A Park, Hee Yi, Hee-Jung Cho, Ki-Hwan Park, Ingyun Hwang, Ho-Chul Shin
Affiliations

Expression of intestinal transporter genes in beagle dogs

Soo-Min Cho et al. Exp Ther Med. 2013 Jan.

Abstract

This study was performed to produce a transcriptional database of the intestinal transporters of beagle dogs. Total RNA was isolated from the duodenum and the expression of various mRNAs was measured using GeneChip(®) oligonucleotide arrays. A total of 124 transporter genes were detected. Genes for fatty acid, peptide, amino acid and glucose and multidrug resistance/multidrug resistance-associated protein (MDR/MRP) transport were expressed at relatively higher levels than the other transporter types. The dogs exhibited abundant mRNA expression of the fatty acid transporters (fatty acid binding proteins, FABPs) FABP1 and FABP2, the ATP-binding cassettes (ABCs) ABCB1A and ABCC2, the amino acid/peptide transporters SLC3A1 and SLC15A1, the glucose transporters SLC5A1, SLC2A2 and SLC2A5, the organic anion transporter SLC22A9 and the phosphate transporters SLC20A1 and SLC37A4. In mice, a similar profile was observed with high expression of the glucose transporters SLC5A1 and SLC2As, the fatty acid transporters FABP1 and FABP2, the MDR/MRP transporters ABCB1A and ABCC2 and the phosphate transporter SLC37A4. However, the overall data reveal diverse transcriptomic profiles of the intestinal transporters of dogs and mice. Therefore, the current database may be useful for comparing the intestinal transport systems of dogs with those of mice to better evaluate xenobiotics.

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Figures

Figure 1
Figure 1
Comparative gene expression of various glucose transporters in the intestines of dogs and mice (n=4). Expression levels were measured as the intensity of the hybridization signal in a GeneChip array and normalized using GeneSpring 7.2. *>2-fold difference in gene expression between the two species (t-test, P<0.01). SLC2, solute carrier family 2 (GLUTs, facilitated glucose transporters); SLC5, solute carrier family 5 (SGLTs, sodium/glucose cotransporters).
Figure 2
Figure 2
Comparative expression of various amino acid and peptide transporter genes in the intestines of dogs and mice (n=4). SLC1, solute carrier family 1 (neuronal/epithelial high affinity glutamate transporters, system Xag); SLC5, solute carrier family 5 (sodium/glucose cotransporters); SLC7, solute carrier family 7 (cationic amino acid transporters, y+ system); CDH17, cadherin 17/Hpt1, SLC15 solute carrier family 15 (oligopeptide transporters); LOC612351, similar to peptide/histidine transporter PHT2.
Figure 3
Figure 3
Comparative expression of various fatty acid transporter genes in the intestines of dogs and mice (n=4). FABP, fatty acid binding protein; SLC27, solute carrier family 27 (fatty acid transporters).
Figure 4
Figure 4
Comparative expression of various nucleobase and nucleoside transporter genes in the intestines of dogs and mice (n=4). SLC23, solute carrier family 23 (nucleobase transporters); SLC28, solute carrier family 28 (sodium-coupled nucleoside transporters, CNTs); SLC29, solute carrier family 29 (nucleoside transporters).
Figure 5
Figure 5
Comparative expression of various MDR and MRP genes in the intestines of dogs and mice (n=4). MDR/MRP, multidrug resistance/multidrug resistance-associated protein; ABCB, ATP-binding cassette sub-family B (MDR/TAP); ABCC, ATP-binding cassette sub-family C (CFTR/MRP); TAP2, transporter 2 ATP-binding cassette sub-family B (MDR/TAP).
Figure 6
Figure 6
Comparative expression of various organic anion transporter genes in the intestines of dogs and mice (n=4). SLC22, solute carrier family 22 (organic cation transporters); LOC612044, similar to SLC22A20; SLCO, solute carrier organic anion transporter family.
Figure 7
Figure 7
Comparative expression of various organic cation transporter genes in the intestines of dogs and mice (n=4). SLC22, solute carrier family 22 (organic cation transporters).
Figure 8
Figure 8
Comparative expression of various phosphate transporter genes in the intestines of dogs and mice (n=4). SLC17, solute carrier family 17 (sodium phosphate transporters); SLC20, solute carrier family 20 (phosphate transporters); SLC34, solute carrier family 34 (sodium phosphate transporters); SLC37, solute carrier family 37 (glycerol-3-phosphate transporters).
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