Poly(A)-specific ribonuclease (PARN): an allosterically regulated, processive and mRNA cap-interacting deadenylase
- PMID: 23496118
- DOI: 10.3109/10409238.2013.771132
Poly(A)-specific ribonuclease (PARN): an allosterically regulated, processive and mRNA cap-interacting deadenylase
Abstract
Deadenylation of eukaryotic mRNA is a mechanism critical for mRNA function by influencing mRNA turnover and efficiency of protein synthesis. Here, we review poly(A)-specific ribonuclease (PARN), which is one of the biochemically best characterized deadenylases. PARN is unique among the currently known eukaryotic poly(A) degrading nucleases, being the only deadenylase that has the capacity to directly interact during poly(A) hydrolysis with both the m(7)G-cap structure and the poly(A) tail of the mRNA. In short, PARN is a divalent metal-ion dependent poly(A)-specific, processive and cap-interacting 3'-5' exoribonuclease that efficiently degrades poly(A) tails of eukaryotic mRNAs. We discuss in detail the mechanisms of its substrate recognition, catalysis, allostery and processive mode of action. On the basis of biochemical and structural evidence, we present and discuss a working model for PARN action. Models of regulation of PARN activity by trans-acting factors are discussed as well as the physiological relevance of PARN.
Similar articles
-
Structural basis of m(7)GpppG binding to poly(A)-specific ribonuclease.Structure. 2009 Feb 13;17(2):276-86. doi: 10.1016/j.str.2008.11.012. Structure. 2009. PMID: 19217398
-
Crystal structure of the RRM domain of poly(A)-specific ribonuclease reveals a novel m(7)G-cap-binding mode.J Mol Biol. 2008 Oct 17;382(4):827-34. doi: 10.1016/j.jmb.2008.07.073. Epub 2008 Jul 31. J Mol Biol. 2008. PMID: 18694759
-
Molecular recognition of mRNA 5' cap by 3' poly(A)-specific ribonuclease (PARN) differs from interactions known for other cap-binding proteins.Biochim Biophys Acta. 2016 Apr;1864(4):331-45. doi: 10.1016/j.bbapap.2016.01.002. Epub 2016 Jan 6. Biochim Biophys Acta. 2016. PMID: 26772900
-
Modulation of poly(A)-specific ribonuclease (PARN): current knowledge and perspectives.Curr Med Chem. 2012;19(28):4838-49. doi: 10.2174/092986712803341539. Curr Med Chem. 2012. PMID: 22834816 Review.
-
Deadenylation: enzymes, regulation, and functional implications.Wiley Interdiscip Rev RNA. 2014 May-Jun;5(3):421-43. doi: 10.1002/wrna.1221. Epub 2014 Feb 12. Wiley Interdiscip Rev RNA. 2014. PMID: 24523229 Review.
Cited by
-
Role of miRNAs in the host-pathogen interaction between sugarcane and Colletotrichum falcatum, the red rot pathogen.Plant Cell Rep. 2021 May;40(5):851-870. doi: 10.1007/s00299-021-02682-9. Epub 2021 Apr 5. Plant Cell Rep. 2021. PMID: 33818644
-
Poly(A)-specific ribonuclease sculpts the 3' ends of microRNAs.RNA. 2019 Mar;25(3):388-405. doi: 10.1261/rna.069633.118. Epub 2018 Dec 27. RNA. 2019. PMID: 30591540 Free PMC article.
-
The H/ACA complex disrupts triplex in hTR precursor to permit processing by RRP6 and PARN.Nat Commun. 2018 Dec 21;9(1):5430. doi: 10.1038/s41467-018-07822-6. Nat Commun. 2018. PMID: 30575725 Free PMC article.
-
Mammalian PNLDC1 is a novel poly(A) specific exonuclease with discrete expression during early development.Nucleic Acids Res. 2016 Oct 14;44(18):8908-8920. doi: 10.1093/nar/gkw709. Epub 2016 Aug 11. Nucleic Acids Res. 2016. PMID: 27515512 Free PMC article.
-
The Interplay between the RNA Decay and Translation Machinery in Eukaryotes.Cold Spring Harb Perspect Biol. 2018 May 1;10(5):a032839. doi: 10.1101/cshperspect.a032839. Cold Spring Harb Perspect Biol. 2018. PMID: 29311343 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
