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Alkaloid profile, antibacterial and allelopathic activities of Lupinus jaimehintoniana B.L. Turner (Fabaceae)

Villa-Ruano Nemesio (Universidad de la Sierra Sur. Guillermo Rojas Mijangos S/N, Ciudad Universitaria, Miahuatlán de Porfirio Díaz, Oaxaca, México)
Pacheco-Hernández Yesenia (Independent Researcher, Miahuatlán de Porfirio Díaz, México)
Rubio-Rosas Efraín (Centro Universitario de Vinculación, Benemérita Universidad Autónoma de Puebla, Puebla, México)
Ruiz-González Nancy (Centro Universitario de Vinculación, Benemérita Universidad Autónoma de Puebla, Puebla, México)
Cruz-Duran Ramiro (Facultad de Ciencias UNAM, Ciudad Universitaria, México DF, Ciudad Universitaria, Del. Coyoacán, México)
Lozoya-Gloria Edmundo (Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato Guanajuato., Mexico)
Zurita-Vásquez Guilibaldo (Universidad de la Sierra Sur. Guillermo Rojas Mijangos, Ciudad Universitaria, Miahuatlán de Porfirio Díaz, Oaxaca, México)
Franco-Monsreal José (Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Centro Histórico, Mérida Yucatán, México)

Herein we describe some aspects of the ethnobotanical use and the first alkaloid profile of Lupinus jaimehintoniana, the 5 to 8 m high arboreous lupine. Five quinolizidine alkaloids identified as sparteine, 5,6-dehydrolupanine, lupanine, nuttalline, and d-thermopsine, were characterized by the respective elution order according to their electronic impact spectra, lupanine being the most abundant in the four different tissues analyzed. Simultaneously, an antibacterial assessment of the four corresponding crude methanolic extracts, as well as the four semi-purified alkaloids was performed on specific Escherichia coli and Agrobacterium tumefaciens strains. These experiments resulted in MIC ranges of 37-61 µg mL-1 and 130-146 µg mL-1, respectively. for both bacterial species. Finally, the allelopathic activity of these extracts on the germination of Lactuca sativa seeds was demonstrated to be in the range of 50-300 µg mL-1 for both semi-purified alkaloid and methanolic extracts.

Keywords: Lupinus jaimehintoniana, alkaloid profile, antibacterial, allelopathic, Mexico