SUMMATIVE TEST BIOTECHNOLOGY 8

Directions: Read the questions carefully. Choose the letter

1 What carries a gene from one organism into a bacterial 14. It is a technique commonly used in laboratories to
cell? separate charged molecules like DNA, RNA and proteins
a. a plasmid b. a restriction enzyme according to their size.
c. electrophoresis gel d. polymerase chain a. Gel Electrophoresis
reaction c. Polymerase Chain Reaction
b. Molecular Scissor
2. The term is defined as the basic physical and functional d. Selection of Prokaryotic Hos
unit of heredity.
a. alleles c. genes 15. When was the Polymerase Chain Reaction discovered?
b. deoxyribonucleic acid d. ribonucleic acid a. 1963
3. Which of the following is a branch of science that is b. 1973
concerned how various traits are inherited? c. 1983
a. biological engineering c. heredity d. 1993
b. genetics d. pathology 16. This refers to the process of manipulating genes for
practical purposes.
4. Which of the following is the process by which a. Genetic engineering
recombinant DNA technology is utilized to alter the genetic b. Karyotyping
makeup of an organism? c. Ligase
a. biological engineering d. Plasmid
c. genetic engineering 17. The circular piece of DNA found in bacteria and
b. biological modification contains genes.
d. genetic modification a. Cell
b. Ligase
5 When did Hindll isolate the first restriction enzyme? c. Plasmid
a. 1960 c. 1990 d. Vector
b. 1980 d. 1970 18. The process by which many copies of a specific gene are
6. Which of the following is the first organism used in DNA made each time the host cell reproduces.
technology experiments? a. Fingerprinting
a. E. coli b. PCR
c. S. cerevisiae c. Gene Cloning
b. E. faecalis d. Recombinant DNA
d. S. typhimurium 19. It is the shape of the DNA.
7. Which of the following contains genes from two different a. Helical
organisms? b. horizontal
a. Altered genome organism c. Parallel
b. Mutant organism d. Vertical
c. Modified organism 20. He discovered the Polymerase Chain Reaction.
d. Transgenic organism a. Hamilton Smith
b. Hindll
8. Which of the following best describes the use of genetic c. Kary Mullis
engineering? d. Werner Abner
a. alter the genetic makeup of an organism 21. These are enzymes used to cut DNA at specific locations
b. generate new hybrid strains based on the nucleotide sequence.
c. improve disease resistance a. Gel electrophoresis
d. all of the above b. Ligase
c. Restriction enzymes
9. Which of the following is the most challenging issue d. PCR
facing genome sequencing or organism modification? 22. It is a genetic engineering tool used to multiply the DNA
a. the availability and stability of DNA exponentially for each of the 25 to 75 cycles.
b. the ethics of using information from genomes at a. Gel electrophoresis
the individual level b. Ligase
c. the inability to develop fast and accurate c. Restriction enzymes
sequencing or modifying techniques. d. PCR
d. all of the above 23. It is a branch of biotechnology deals with the study and
10. Which of the laboratory technique is used to amplify a investigation of genomic information from trace evidence
single copy or a few copies of a segment of DNA across found at crime scenes.
several orders of magnitude, generating thousands to a. Agricultural biotechnology
millions of copies of a particular DNA sequence? b. Forensic biotechnology
a. Agarose Gel Electrophoresis c. c. Industrial biotechnology
Polymerase Chain Reaction d. Medical biotechnology
b. Molecular Scissor d. Restriction 24. This was the name given to the first mouse used to
Endonuclease study cancer.
a. Mighty mouse
11. The enzymes that make one incision on each of the two b. Mousegenic
strands of DNA at specific locations based on the nucleotide c. Oncomouse
sequence are known as ______________. d. Supermouse
a. DNA Polymerase I c. Primase 25. This is the process where mice are used to study gene
b. Helicase d. Restriction Endonuclease function by purposely turning off specific genes.
a. Apoptosis
12.Which of the following scientists discovered the b. Cell knockout
Polymerase Chain Reaction? c. Gene knockout
a. Hamilton Smith c. Kary Mullis d. Genetosis
b. Hindll d. Werner Abner 26. Which genetic engineering process contains foreign DNA
that has been introduced using biotechnology?
13. Which of the following is the branch of biotechnology a. Gene cloning
that deals with the study and investigation of genomic b. GMO
information from trace evidence found at crime scene? c. Nuclear cloning
a. biotechnology d. Transgenic organism
b. Forensics biotechnology
c. Industrial biotechnology
d. Medical biotechnology
27. Who developed the process of nuclear cloning?
a. Hamilton Smith
b. Hans Spemman 40. What organism is being used to transfer foreign genetic
c. Hindll material into a cell?
d. Kary Mullis a. DNA
28.When was the first nuclear cloning conceived? b. Plasmid
a. 1928 c. Restriction enzymes
b. 1929 d. Vector
c. 1930 41. Which enzyme is being used to amplify the DNA?
d. 1931 a. Endonuclease
b. DNA Helicase
29. Which organism was modified to produce FDA-approved c. DNA Ligase d.
human antithrombin (ATryn). DNA Polymerase
a. Cat 42. The following statements are all TRUE EXCEPT
b. Frog a. The DNA is located in the nucleus of the cell.
c. Goat b. Recombinant DNA is a combination of two
d. Sheep different strands of DNA.
30. What vitamin is present in Golden rice? c. DNA Ligase is used to amplify a single copy or a
a. Vitamin A few copies of DNA.
b. Vitamin B d. In Agarose Gel Electrophoresis the negatively
c. Vitamin C charged DNA travels to the positive electrode and is
d. Vitamin D separated out based on size.
31. Which refers to the combination of two DNA strands For question nos. 43-49, Organize the steps in Recombinant
that are constructed artificially? DNA Technology. Use letters a to g
a. Genetic Material __43. Amplification Using PCR
b. Recombinant Cells __44. Isolation of Genetic Material
c. Recombinant DNA __45. Insertion of Recombinant DNA into Host
d. Restriction Enzymes __46. Obtaining or culturing the Foreign Gene product.
32. Which among the following is the Blueprint of Life? __47. Ligation of DNA Molecules.
a. Cell __48. Restriction Enzymes Digestion
b. DNA __49. Isolation of Recombinant Cells
c. Protein
d. RNA 50. Which of the following refers to chemically synthesized
33. Which refers to the small accessory ring of the DNA in oligonucleotides that are complementary to a region of the
some bacteria? DNA?
a. Interferon a. Agarose Gel b.
b. Plasmid Primers
c. Restriction enzymes c. Restriction Enzymes d.
d. Vector Vectors
34. What molecule is being used to cut a specific area of the 51. Which of the following is TRUE?
DNA? a. DNA is positively charged.
a. Agarose Gel b. Ligase is used to isolate the genetic material.
b. Plasmid c. Thermal Cycler cuts fragment of DNA and the
c. Recombinant Cells vector.
d. Restriction enzymes d. Gel Electrophoresis separates the DNA Molecule
35. An organism that contains genetic material from two according to size
different organisms is called ___________.
a. Clone For question nos. 52-55. Identify the steps in Recombinant
DNA Technology that are being described in each statement.
b. GMO The choices are as follows:
c. Mutant a. Amplification Using PCR
d. Restriction enzymes b. Isolation of Genetic Material
36. What technique is being used to make a million copies c. Insertion of Recombinant DNA into Host
of a sample DNA? d. Obtaining or culturing the Foreign Gene product.
a. DNA Fingerprinting e. Ligation of DNA Molecules.
b. Gel Electrophoresis f. Restriction Enzymes Digestion
c. Gene Therapy g. Isolation of Recombinant Cells
d. Polymerase Chain Reaction
37. Which among the following does NOT describe the __52. Marker gene of plasmid vectors is employed.
principle of Gel Electrophoresis? __53. The aim is to produce a desirable protein expression.
a. DNA fragments are separated on the basis of size. __54. The DNA molecule is separated and purified using
b. The DNA fragments will move towards the enzymes.
negative charge. __55. The recombinant DNA is introduced into a recipient
c. The smallest DNA fragments will move faster host cell.
than the larger DNA fragments.
d. DNA fragments are injected into wells and an
electric current is applied along with the gel.
38. What equipment is used to amplify the DNA?
a. Centrifuge Machine
b. Microscope
c. Pipette
d. Thermal Cycler
39. Which among the following serves as a starting point for
DNA synthesis?
a. DNA polymerase
b. Primer
c. Restriction enzymes
d. Taq polymerase